Hotspot

trnC-GCA-petN

LSC · 28569-29424 · 856 bp

Back to Oxyspora

Pi 0.00954

MarkerSeek score 61.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 856
variable_sites: 16
pi: 0.00954
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	914-919 bp	1.0	81.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CCTTTAGAGTCCACTTCGCC	50.0	55.0	58.1	58.0	973-978 bp	1.0	81.0
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CTTTAGAGTCCACTTCGCCC	50.0	55.0	58.1	58.0	972-977 bp	1.0	81.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	908-913 bp	1.0	79.7
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGACGACCATTAAAGCAGCC	50.0	50.0	58.1	58.3	923-928 bp	1.0	79.1