Hotspot

trnH-GUG-psbA

LSC · 76-304 · 229 bp

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Pi 0.0507

MarkerSeek score 81.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 23
pi: 0.0507
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	296-302 bp	1.0	85.7
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	346-352 bp	1.0	84.9
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	360-366 bp	1.0	84.6
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	359-365 bp	1.0	84.6
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	361-367 bp	1.0	84.6