Hotspot

clpP

LSC · 71393-73706 · 2314 bp

Back to Oxyria

Pi 0.0108
MarkerSeek score 49.80
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2314
variable_sites
25
pi
0.0108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 ACCCTAGTACAGGTTCCTCG GCCGCCATATTTGTTCATCG 55.0 50.0 57.9 58.0 2775-2777 bp 1.0 79.6
clpP_p2 AGTCGCACATACACCCTAGT GCCGCCATATTTGTTCATCG 50.0 50.0 58.2 58.0 2787-2789 bp 1.0 79.6
clpP_p3 ACCCTAGTACAGGTTCCTCG TATCAACGGACCCCTGTTCT 55.0 50.0 57.9 58.1 2954-2956 bp 1.0 79.5
clpP_p4 GCTACCAATGGGAACTTGCT GCCGCCATATTTGTTCATCG 50.0 50.0 58.2 58.0 2986-2988 bp 1.0 79.4
clpP_p5 ACCCTAGTACAGGTTCCTCG GCAAATGCCGCCATATTTGT 55.0 45.0 57.9 58.1 2781-2783 bp 1.0 79.4