Hotspot

rps15-ycf1

IRa · 129720-130000 · 281 bp

Back to Oxyria

Pi 0.0262
MarkerSeek score 57.30
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
IRa
length_bp
281
variable_sites
7
pi
0.0262
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AAGTCGTTGACGTTTTCCCA TGCAATTGAAATGGCTGCTG 45.0 45.0 58.0 57.9 1169-1183 bp 1.0 80.9
rps15-ycf1_p2 AGTCGTTGACGTTTTCCCAA TGCAATTGAAATGGCTGCTG 45.0 45.0 58.0 57.9 1168-1182 bp 1.0 80.9
rps15-ycf1_p3 TGGGTGTTGAGGTCCCATAT TGCAATTGAAATGGCTGCTG 50.0 45.0 58.0 57.9 1411-1425 bp 1.0 80.7
rps15-ycf1_p4 AGTCGTTGACGTTTTCCCAA GATTATGCGCTGTCCGTACT 45.0 50.0 58.0 57.9 1124-1138 bp 1.0 80.5
rps15-ycf1_p5 TGGGTGTTGAGGTCCCATAT GATTATGCGCTGTCCGTACT 50.0 50.0 58.0 57.9 1367-1381 bp 1.0 80.3