Hotspot

petL-petG

LSC · 66838-66998 · 161 bp

Back to Oxyria

Pi 0.0186
MarkerSeek score 62.10
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
161
variable_sites
3
pi
0.0186
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 AGCTCCTTCATGCCTACTCT CAACTGATCACCACGCCTAT 50.0 50.0 57.8 57.7 371-374 bp 1.0 76.7
petL-petG_p2 AGCTCCTTCATGCCTACTCT AAGGTCCAACTGATCACCAC 50.0 50.0 57.8 57.4 377-380 bp 1.0 74.7
petL-petG_p3 TGGTCTGAGCAAGATACGAC CAACTGATCACCACGCCTAT 50.0 50.0 57.1 57.7 294-297 bp 1.0 70.8
petL-petG_p4 AGCTCCTTCATGCCTACTCT AAAGGTCCAACTGATCACCAC 50.0 47.6 57.8 58.1 378-381 bp 1.0 70.2
petL-petG_p5 AGCTCCTTCATGCCTACTCT CAAAGGTCCAACTGATCACCA 50.0 47.6 57.8 58.1 379-382 bp 1.0 70.1