Hotspot

psbE-petL

LSC · 65560-66741 · 1182 bp

Back to Oxyria

Pi 0.00511
MarkerSeek score 54.60
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1182
variable_sites
6
pi
0.00511
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GTCGTATCTTGCTCAGACCA 50.0 50.0 57.9 57.1 1367-1374 bp 1.0 72.8
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1290-1297 bp 1.0 72.0
psbE-petL_p3 ACGAATAACCAACCCGCAAT GTCGTATCTTGCTCAGACCA 45.0 50.0 58.2 57.1 1376-1383 bp 1.0 71.9
psbE-petL_p4 TGCTGACGAATAACCAACCC GTCGTATCTTGCTCAGACCA 50.0 50.0 58.2 57.1 1381-1388 bp 1.0 71.7
psbE-petL_p5 CGAATAACCAACCCGCAATG GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1375-1382 bp 1.0 71.7