Hotspot

rps4-trnT-UGU

LSC · 47797-48359 · 563 bp

Back to Oxyria

Pi 0.00888
MarkerSeek score 63.10
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
563
variable_sites
5
pi
0.00888
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 CCCCCAGACGTCGTATTTTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.9 57.9 675-679 bp 1.0 78.7
rps4-trnT-UGU_p2 CCCCCAGACGTCGTATTTTT GATGGTCATCGGTTCGACTC 50.0 55.0 57.9 58.2 644-648 bp 1.0 78.5
rps4-trnT-UGU_p3 GGAACGCGATGGGTTTCTAA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.3 57.9 731-735 bp 1.0 77.3
rps4-trnT-UGU_p4 GGAACGCGATGGGTTTCTAA GATGGTCATCGGTTCGACTC 50.0 55.0 58.3 58.2 700-704 bp 1.0 77.1
rps4-trnT-UGU_p5 CCCCCAGACGTCGTATTTTT TTTATGTGAGCCCGCTTAGC 50.0 50.0 57.9 58.3 694-698 bp 1.0 77.1