Hotspot

psbI-trnS-GCU

LSC · 8559-8663 · 105 bp

Back to Oxyria

Pi 0.019
MarkerSeek score 63.50
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
105
variable_sites
2
pi
0.019
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAAGA GTCATTCGTACCGAGGGTTC 50.0 55.0 57.1 58.1 166 bp 1.0 72.8
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAAGA TAGGGAGAGATGGCTGAGTG 50.0 55.0 57.1 57.9 220 bp 1.0 72.6
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAAGAA GTCATTCGTACCGAGGGTTC 47.6 55.0 57.8 58.1 166 bp 1.0 70.5
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAAGAA TAGGGAGAGATGGCTGAGTG 47.6 55.0 57.8 57.9 220 bp 1.0 70.3
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGA AGTCATTCGTACCGAGGGTT 50.0 50.0 57.1 58.4 167 bp 1.0 69.9