Hotspot

trnK-UUU-rps16

LSC · 4226-4850 · 625 bp

Back to Oxyria

Pi 0.0048
MarkerSeek score 61.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
625
variable_sites
3
pi
0.0048
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AACTCTACCGATGGTGTGGA CGCTCAACCTACTGGAACTG 50.0 55.0 58.1 58.3 815 bp 1.0 77.5
trnK-UUU-rps16_p2 TGGTGTGGACGAATTCCTTG CGCTCAACCTACTGGAACTG 50.0 55.0 58.1 58.3 804 bp 1.0 77.1
trnK-UUU-rps16_p3 AACTCTACCGATGGTGTGGA ATTTTCTTGAAAGGGGCGCT 50.0 45.0 58.1 58.4 831 bp 1.0 76.8
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT CGCTCAACCTACTGGAACTG 50.0 55.0 58.2 58.3 747 bp 1.0 76.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CGCTCAACCTACTGGAACTG 50.0 55.0 58.2 58.3 746 bp 1.0 76.7