Hotspot

accD

LSC · 57041-58918 · 1878 bp

Back to Oxandra

Pi 0.0151

MarkerSeek score 60.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1878
variable_sites: 63
pi: 0.0151
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ACGAAATAGATGTATGATAATCCGC	AAGCAATGGACAGTCTTGGC	36.0	50.0	57.2	58.7	1862-1970 bp	1.0	46.6
accD_p2	CGAAATAGATGTATGATAATCCGCT	AAGCAATGGACAGTCTTGGC	36.0	50.0	57.0	58.7	1861-1969 bp	1.0	46.6
accD_p3	ACGAAATAGATGTATGATAATCCGC	AGCAATGGACAGTCTTGGC	36.0	52.6	57.2	58.0	1861-1969 bp	1.0	46.6
accD_p4	CGAAATAGATGTATGATAATCCGC	AAGCAATGGACAGTCTTGGC	37.5	50.0	55.7	58.7	1861-1969 bp	1.0	46.6
accD_p5	CGAAATAGATGTATGATAATCCGCT	AGCAATGGACAGTCTTGGC	36.0	52.6	57.0	58.0	1860-1968 bp	1.0	46.6