Hotspot

trnL-CAA-ycf2

IRa · 148563-149604 · 1042 bp

Back to Oxandra

Pi 0.0118

MarkerSeek score 61.70

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 1042
variable_sites: 25
pi: 0.0118
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	AGGCATTGTGGAGAAAGAGA	50.0	45.0	58.0	56.4	1203-1226 bp	1.0	69.1
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	AGGCATTGTGGAGAAAGAGA	50.0	45.0	58.0	56.4	1205-1228 bp	1.0	69.1
trnL-CAA-ycf2_p3	AAATGGTAGACACGCGAGAC	AGGAGAAGGATTCCCCATTC	50.0	50.0	58.0	56.3	1136-1159 bp	1.0	67.8
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	AGGAGAAGGATTCCCCATTC	50.0	50.0	58.0	56.3	1138-1161 bp	1.0	67.8
trnL-CAA-ycf2_p5	TGGTGAAATGGTAGACACGC	AGGCATTGTGGAGAAAGAGA	50.0	45.0	58.2	56.4	1208-1231 bp	1.0	67.6