Hotspot

ccsA-ndhD

SSC · 119589-119807 · 219 bp

Back to Oxandra

Pi 0.024

MarkerSeek score 69.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 219
variable_sites: 14
pi: 0.024
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGCTATTTTGGGGTCA	TCCCGATTTCCTTCTCTCACT	40.9	47.6	57.2	58.2	361-367 bp	1.0	54.7
ccsA-ndhD_p2	TGGATATGCTATTTTGGGGTCA	TCAGTTGACAAGGCAGAAACT	40.9	42.9	57.2	57.7	339-345 bp	1.0	54.3
ccsA-ndhD_p3	TGGATATGCTATTTTGGGGTCA	TCCCGATTTCCTTCTCTCAC	40.9	50.0	57.2	56.6	361-367 bp	1.0	53.3
ccsA-ndhD_p4	TGGATATGCTATTTTGGGGTCA	CCCGATTTCCTTCTCTCACT	40.9	50.0	57.2	56.6	360-366 bp	1.0	53.3
ccsA-ndhD_p5	TGGATATGCTATTTTGGGGTCAA	TCCCGATTTCCTTCTCTCACT	39.1	47.6	57.9	58.2	361-367 bp	1.0	52.1