Hotspot

rpl32-trnL-UAG

SSC · 117249-118445 · 1197 bp

Back to Oxandra

Pi 0.0231

MarkerSeek score 67.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1197
variable_sites: 63
pi: 0.0231
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTTTGTCCGACAAACAGGT	AGCGTGTCTACCGATTTCAC	40.0	50.0	56.0	58.0	1248-1427 bp	1.0	68.6
rpl32-trnL-UAG_p2	TTTTTGTCCGACAAACAGGT	CAGCGTGTCTACCGATTTCA	40.0	50.0	56.0	58.0	1249-1428 bp	1.0	68.5
rpl32-trnL-UAG_p3	GCTAAATCTATTGCTACCGGGT	AGCGTGTCTACCGATTTCAC	45.5	50.0	58.0	58.0	1283-1462 bp	1.0	68.3
rpl32-trnL-UAG_p4	GCTAAATCTATTGCTACCGGGT	CAGCGTGTCTACCGATTTCA	45.5	50.0	58.0	58.0	1284-1463 bp	1.0	68.3
rpl32-trnL-UAG_p5	TTTTTGTCCGACAAACAGGT	TTTTAGAGATGCCGCCACTC	40.0	50.0	56.0	58.0	1308-1487 bp	1.0	68.2