Hotspot

ycf2-trnL-CAA

IRb · 97545-98586 · 1042 bp

Back to Oxandra

Pi 0.0118

MarkerSeek score 61.70

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 1042
variable_sites: 25
pi: 0.0118
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	AGGCATTGTGGAGAAAGAGA	AAATGGTAGACACGCGAGAC	45.0	50.0	56.4	58.0	1203-1226 bp	1.0	69.1
ycf2-trnL-CAA_p2	AGGCATTGTGGAGAAAGAGA	TGAAATGGTAGACACGCGAG	45.0	50.0	56.4	58.0	1205-1228 bp	1.0	69.1
ycf2-trnL-CAA_p3	AGGAGAAGGATTCCCCATTC	AAATGGTAGACACGCGAGAC	50.0	50.0	56.3	58.0	1136-1159 bp	1.0	67.8
ycf2-trnL-CAA_p4	AGGAGAAGGATTCCCCATTC	TGAAATGGTAGACACGCGAG	50.0	50.0	56.3	58.0	1138-1161 bp	1.0	67.8
ycf2-trnL-CAA_p5	AGGCATTGTGGAGAAAGAGA	TGGTGAAATGGTAGACACGC	45.0	50.0	56.4	58.2	1208-1231 bp	1.0	67.6