Hotspot

rpoA-rps11

IRb · 82815-82941 · 127 bp

Back to Oxandra

Pi 0.0682

MarkerSeek score 76.60

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: IRb
length_bp: 127
variable_sites: 17
pi: 0.0682
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	CGTTGCGAGTTCCATACAGA	GTCAGGATAAAGGGTGCTGG	50.0	55.0	58.0	58.0	533-607 bp	1.0	88.1
rpoA-rps11_p2	CGCTTACTGTCGGTTCTTGA	TCATGTTCAAGCGGGTTTCA	50.0	45.0	57.9	58.0	481-555 bp	1.0	87.4
rpoA-rps11_p3	TCGCTTACTGTCGGTTCTTG	TCATGTTCAAGCGGGTTTCA	50.0	45.0	57.9	58.0	482-556 bp	1.0	87.3
rpoA-rps11_p4	GTCGCTTACTGTCGGTTCTT	TCATGTTCAAGCGGGTTTCA	50.0	45.0	57.9	58.0	483-557 bp	1.0	87.3
rpoA-rps11_p5	CGTTGCGAGTTCCATACAGA	TCATGTTCAAGCGGGTTTCA	50.0	45.0	58.0	58.0	720-794 bp	1.0	87.2