Hotspot

trnF-GAA-ndhJ

LSC · 50266-50972 · 707 bp

Back to Oxandra

Pi 0.0104

MarkerSeek score 61.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 20
pi: 0.0104
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	869-882 bp	1.0	83.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	833-846 bp	1.0	82.9
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	841-854 bp	1.0	82.7
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	805-818 bp	1.0	82.5
trnF-GAA-ndhJ_p5	AGAATGGTCGGGATAGCTCA	ATCCACGCCTGAAACGTATC	50.0	50.0	57.9	58.1	878-891 bp	1.0	82.4