Hotspot

trnT-GGU-psbD

LSC · 33398-34832 · 1435 bp

Back to Oxandra

Pi 0.0267

MarkerSeek score 71.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1435
variable_sites: 114
pi: 0.0267
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CTGTTCTTGAACAAGCCCCT	ACAAAAACAGCAATTGGGGC	50.0	45.0	58.0	58.0	2089-2096 bp	0.857143	77.2
trnT-GGU-psbD_p2	CTGTTCTTGAACAAGCCCCT	CAAAAACAGCAATTGGGGCA	50.0	45.0	58.0	58.0	2088-2095 bp	0.857143	77.2
trnT-GGU-psbD_p3	TTGTCCGTCAGAAAAAGGGG	ACAAAAACAGCAATTGGGGC	50.0	45.0	58.0	58.0	2523-2528 bp	0.857143	77.2
trnT-GGU-psbD_p4	GTTCAGGGTACGAGTCGAAC	ACAAAAACAGCAATTGGGGC	55.0	45.0	58.0	58.0	2301-2308 bp	0.857143	77.0
trnT-GGU-psbD_p5	CCGTCAGAAAAAGGGGTCAA	ACAAAAACAGCAATTGGGGC	50.0	45.0	58.0	58.0	2523-2524 bp	0.428571	57.8