Hotspot

trnE-UUC-trnT-GGU

LSC · 32584-33325 · 742 bp

Back to Oxandra

Pi 0.0312

MarkerSeek score 75.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 70
pi: 0.0312
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TAGGGCATTTCTGGAGGACA	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1063-1141 bp	1.0	88.0
trnE-UUC-trnT-GGU_p2	CCATGATCAACTTCCCCTCG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	892-970 bp	0.857143	82.3
trnE-UUC-trnT-GGU_p3	TAGGGCATTTCTGGAGGACA	AAATTCTTGCGTGGTCCAGT	50.0	45.0	58.0	58.0	1550-1619 bp	0.857143	77.6
trnE-UUC-trnT-GGU_p4	TGCGATTATTGCCACATCGA	GAACCGATGACTTACGCCTT	45.0	50.0	58.1	58.0	1171-1249 bp	0.714286	75.0
trnE-UUC-trnT-GGU_p5	CCATGATCAACTTCCCCTCG	AAATTCTTGCGTGGTCCAGT	55.0	45.0	58.0	58.0	1381-1448 bp	0.714286	71.0