Hotspot

petN-psbM

LSC · 29587-30703 · 1117 bp

Back to Oxandra

Pi 0.0213
MarkerSeek score 62.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1117
variable_sites
55
pi
0.0213
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ATGGGGAAGAAGTGGACTCT ACGTAAAAACAGTCAGTCAA 50.0 35.0 57.6 53.0 1167-1187 bp 1.0 44.2
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT ACGTAAAAACAGTCAGTCAA 50.0 35.0 58.2 53.0 1228-1248 bp 1.0 44.0
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT ACGTAAAAACAGTCAGTCAAA 50.0 33.3 58.2 53.9 1228-1248 bp 1.0 44.0
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT ACGTAAAAACAGTCAGTCAAAATGA 50.0 32.0 58.2 57.9 1228-1248 bp 1.0 44.0
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT ACGTAAAAACAGTCAGTCAAAA 50.0 31.8 58.2 54.8 1228-1248 bp 1.0 44.0