Hotspot

rps15-ycf1

SSC · 121764-122181 · 418 bp

Back to Oxalis

Pi 0.0858
MarkerSeek score 81.30
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
418
variable_sites
92
pi
0.0858
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCCCAACGCGTTACATAAGG GACATCAAAAGGCGGCTAGA 50.0 50.0 58.0 58.0 1362-1396 bp 0.222222 52.1
rps15-ycf1_p2 TCCCCCGGTGTAAATAACCT GACATCAAAAGGCGGCTAGA 50.0 50.0 58.0 58.0 1301-1335 bp 0.222222 52.1
rps15-ycf1_p3 TCCCAACGCGTTACATAAGG AGACATCAAAAGGCGGCTAG 50.0 50.0 58.0 58.0 1363-1397 bp 0.222222 52.0
rps15-ycf1_p4 TCCCCCGGTGTAAATAACCT AGACATCAAAAGGCGGCTAG 50.0 50.0 58.0 58.0 1302-1336 bp 0.222222 52.0
rps15-ycf1_p5 GTGCACCAAGATCTGCCATA GACATCAAAAGGCGGCTAGA 50.0 50.0 58.0 58.0 1532-1566 bp 0.222222 51.5