Hotspot

ndhF-trnL-UAG

SSC · 112061-112754 · 694 bp

Back to Oxalis

Pi 0.0758
MarkerSeek score 80.20
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
694
variable_sites
126
pi
0.0758
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 GTGGATCGATTAAGCAGCCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 989-1198 bp 0.666667 78.2
ndhF-trnL-UAG_p2 TAGGAAAAGCCCACATACGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 856-1054 bp 0.222222 52.1
ndhF-trnL-UAG_p3 TAGGAAAAGCCCACATACGC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 857-1055 bp 0.222222 52.1
ndhF-trnL-UAG_p4 TAGGAAAAGCCCACATACGC TGCCCTTTTTGGACGAATCA 50.0 45.0 58.0 58.0 1150-1348 bp 0.222222 51.4
ndhF-trnL-UAG_p5 TAGGAAAAGCCCACATACGC TCATGCCCTTTTTGGACGAA 50.0 45.0 58.0 58.0 1153-1351 bp 0.222222 51.3