Hotspot

rpl36-rps8

LSC · 79677-80157 · 481 bp

Back to Oxalis

Pi 0.0696
MarkerSeek score 81.10
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
481
variable_sites
81
pi
0.0696
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 CCCTCTTCTTGTCCCCCTAA TAGAATTTTGGGCGGGATGG 55.0 50.0 58.0 57.9 986-1010 bp 1.0 91.2
rpl36-rps8_p2 CCCTAAATCCACACGTACCG TAGAATTTTGGGCGGGATGG 55.0 50.0 58.1 57.9 972-996 bp 1.0 90.9
rpl36-rps8_p3 CCTAAATCCACACGTACCGG TAGAATTTTGGGCGGGATGG 55.0 50.0 58.1 57.9 971-995 bp 1.0 90.9
rpl36-rps8_p4 CCCTCTTCTTGTCCCCCTAA TCGTTTCATTAGCGCCAGAA 55.0 45.0 58.0 57.9 1510-1523 bp 0.222222 50.2
rpl36-rps8_p5 CCTAAATCCACACGTACCGG TCGTTTCATTAGCGCCAGAA 55.0 45.0 58.1 57.9 1495-1508 bp 0.222222 50.0