Hotspot

ndhC-trnC-ACA

LSC · 51218-52258 · 1041 bp

Back to Oxalis

Pi 0.0735
MarkerSeek score 80.50
Primer available yes
Label ndhC-trnC-ACA

Feature Metadata

label_name
ndhC-trnC-ACA
feature_type
igs
region
LSC
length_bp
1041
variable_sites
186
pi
0.0735
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.95
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnC-ACA_p1 GCCCAGAAAATATCATATTCGT GGTTCGAATCCGTATAGCCC 36.4 55.0 54.4 57.9 1018-1098 bp 1.0 52.0
ndhC-trnC-ACA_p2 GCCCAGAAAATATCATATTCGT CGGTTCGAATCCGTATAGCC 36.4 55.0 54.4 58.3 1019-1099 bp 1.0 52.0
ndhC-trnC-ACA_p3 TCATATTCGTAAAGCAGAAACA GGTTCGAATCCGTATAGCCC 31.8 55.0 53.9 57.9 1006-1086 bp 1.0 52.0
ndhC-trnC-ACA_p4 TCATATTCGTAAAGCAGAAACA CGGTTCGAATCCGTATAGCC 31.8 55.0 53.9 58.3 1007-1087 bp 1.0 52.0
ndhC-trnC-ACA_p5 GCCCAGAAAATATCATATTCG GGTTCGAATCCGTATAGCCC 38.1 55.0 52.6 57.9 1018-1098 bp 1.0 52.0