Hotspot

trnF-GAA-ndhJ

LSC · 48830-49543 · 714 bp

Back to Oxalis

Pi 0.0559
MarkerSeek score 84.90
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
714
variable_sites
92
pi
0.0559
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA ATCCACGCCTGAAACGTATC 55.0 50.0 58.0 58.1 874-914 bp 1.0 90.8
trnF-GAA-ndhJ_p2 GGGATAGCTCAGCTGGTAGA CGTTCCCAATGTGCCTATGA 55.0 50.0 58.0 58.0 1094-1134 bp 1.0 90.1
trnF-GAA-ndhJ_p3 GGGATAGCTCAGCTGGTAGA ATCTACGTTCCCAATGTGCC 55.0 50.0 58.0 58.0 1099-1139 bp 1.0 90.0
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA CGTTCCCAATGTGCCTATGA 50.0 50.0 58.1 58.0 1058-1098 bp 1.0 90.0
trnF-GAA-ndhJ_p5 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 856-882 bp 0.222222 49.0