Hotspot

psaA-ycf3

LSC · 42682-43342 · 661 bp

Back to Oxalis

Pi 0.0454
MarkerSeek score 82.80
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
661
variable_sites
76
pi
0.0454
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 ATGCCACTCAGCCAAAGAAA GCGCAGAATTGGTTGAAGAC 45.0 50.0 58.0 58.0 955-997 bp 1.0 90.0
psaA-ycf3_p2 ATGCCACTCAGCCAAAGAAA CCGTATGAGGCAGGAAACTC 45.0 55.0 58.0 58.1 1133-1175 bp 1.0 88.6
psaA-ycf3_p3 ATGCCACTCAGCCAAAGAAA GAATTAACTGCCGAGCCGTA 45.0 50.0 58.0 58.1 1156-1190 bp 0.666667 71.9
psaA-ycf3_p4 ATGCCACTCAGCCAAAGAAA GATCCCCCTAAAGCGTTGAG 45.0 55.0 58.0 58.0 1446-1464 bp 0.555556 69.1
psaA-ycf3_p5 ATGCCACTCAGCCAAAGAAA ATCAAGCCGCTGAGTATTGG 45.0 50.0 58.0 58.0 1022-1043 bp 0.222222 50.0