Hotspot

psbZ-trnG-GCC

LSC · 36882-37310 · 429 bp

Back to Oxalis

Pi 0.0704
MarkerSeek score 81.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
429
variable_sites
78
pi
0.0704
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CCGGTACATCATTATGGATTGGA TACCATTCGACTATATCCGC 43.5 45.0 58.1 54.3 500-585 bp 1.0 52.0
psbZ-trnG-GCC_p2 TCCGGTACATCATTATGGATTGG TACCATTCGACTATATCCGC 43.5 45.0 58.1 54.3 501-586 bp 1.0 52.0
psbZ-trnG-GCC_p3 CCGGTACATCATTATGGATTGGA TTACCATTCGACTATATCCGC 43.5 42.9 58.1 55.1 501-586 bp 1.0 52.0
psbZ-trnG-GCC_p4 TCCGGTACATCATTATGGATTGG TTACCATTCGACTATATCCGC 43.5 42.9 58.1 55.1 502-587 bp 1.0 52.0
psbZ-trnG-GCC_p5 CCGGTACATCATTATGGATTGG TACCATTCGACTATATCCGC 45.5 45.0 56.7 54.3 500-585 bp 1.0 52.0