Hotspot

trnT-GGU-psbD

LSC · 32077-33585 · 1509 bp

Back to Oxalis

Pi 0.0619
MarkerSeek score 88.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1509
variable_sites
217
pi
0.0619
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
88.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1409-1738 bp 1.0 89.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1412-1741 bp 1.0 89.3
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG CCCTTCGTAACCAGTCATCC 55.0 55.0 58.1 57.7 1393-1722 bp 1.0 89.0
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA CCCTTCGTAACCAGTCATCC 50.0 55.0 58.1 57.7 1396-1725 bp 1.0 88.8
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG GCAATAAACCGGACCAACCT 55.0 50.0 58.1 58.5 1429-1758 bp 1.0 87.8