Hotspot

trnE-UUC-trnT-GGU

LSC · 31252-32004 · 753 bp

Back to Oxalis

Pi 0.0633
MarkerSeek score 82.00
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
753
variable_sites
120
pi
0.0633
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.93
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CCAGCGTAGACATATTGCCA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 946-1034 bp 1.0 90.0
trnE-UUC-trnT-GGU_p2 AATCGTCGTGTTGAAACCGA GAACCGATGACTTACGCCTT 45.0 50.0 58.1 58.0 1397-1418 bp 0.222222 53.3
trnE-UUC-trnT-GGU_p3 AATCGTCGTGTTGAAACCGA CCATGGCGTTACTCTACCAC 45.0 55.0 58.1 58.1 1376-1397 bp 0.222222 52.9
trnE-UUC-trnT-GGU_p4 AATCGTCGTGTTGAAACCGA TGGCGTTACTCTACCACTGA 45.0 50.0 58.1 58.1 1373-1394 bp 0.222222 52.7
trnE-UUC-trnT-GGU_p5 AATCGTCGTGTTGAAACCGA TGTGGAGGAAGGGAATAGCA 45.0 50.0 58.1 58.0 1986-2019 bp 0.222222 52.3