Hotspot

trnD-GUC-trnY-GUA

LSC · 30580-31036 · 457 bp

Back to Oxalis

Pi 0.0685
MarkerSeek score 82.00
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
457
variable_sites
74
pi
0.0685
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 485-527 bp 1.0 86.3
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 484-526 bp 1.0 86.3
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 506-548 bp 1.0 85.4
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 505-547 bp 1.0 85.2
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA CGGACTGTAAATTCGTTGGC 50.0 50.0 58.5 57.5 501-543 bp 1.0 84.0