Hotspot

psbM-trnD-GUC

LSC · 29698-30505 · 808 bp

Back to Oxalis

Pi 0.0655
MarkerSeek score 89.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
808
variable_sites
132
pi
0.0655
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGTAAGAAGGCAGTAGGGACT TTCAATTGGTCAGAGCACCG 47.6 50.0 57.5 58.5 917-940 bp 1.0 76.4
psbM-trnD-GUC_p2 AGTAAGAAGGCAGTAGGGACT GTTCAATTGGTCAGAGCACC 47.6 50.0 57.5 57.0 918-941 bp 1.0 72.2
psbM-trnD-GUC_p3 AGTAAGAAGGCAGTAGGGAC TTCAATTGGTCAGAGCACCG 50.0 50.0 56.0 58.5 917-940 bp 1.0 71.8
psbM-trnD-GUC_p4 GTAAGAAGGCAGTAGGGACT TTCAATTGGTCAGAGCACCG 50.0 50.0 56.0 58.5 916-939 bp 1.0 71.8
psbM-trnD-GUC_p5 AGGGACTAAAATGAACAGTGCA TTCAATTGGTCAGAGCACCG 40.9 50.0 57.9 58.5 903-926 bp 1.0 71.4