Hotspot

trnC-GCA-petN

LSC · 27892-28872 · 981 bp

Back to Oxalis

Pi 0.0766
MarkerSeek score 84.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
981
variable_sites
188
pi
0.0766
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GAGGTGTGTAAGAAGGCTCG CGCATTTCCCCGAGTTCTTA 55.0 50.0 58.0 58.0 1990-2004 bp 0.444444 66.4
trnC-GCA-petN_p2 TTTTGTATCGTTTTGGCGGC TTAAAGCAGCCCAAGCAAGA 45.0 45.0 57.9 58.0 1100-1103 bp 0.222222 56.6
trnC-GCA-petN_p3 ACAATCAATCGAAGGGACGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.0 58.0 1182-1252 bp 0.222222 56.3
trnC-GCA-petN_p4 GAGGTGTGTAAGAAGGCTCG TTAAAGCAGCCCAAGCAAGA 55.0 45.0 58.0 58.0 1749 bp 0.111111 44.4
trnC-GCA-petN_p5 ACAATCAATCGAAGGGACGG CGCATTTCCCCGAGTTCTTA 50.0 50.0 58.0 58.0 1437 bp 0.111111 44.1