Hotspot

rpoB-trnC-GCA

LSC · 26628-27820 · 1193 bp

Back to Oxalis

Pi 0.0535
MarkerSeek score 83.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1193
variable_sites
173
pi
0.0535
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TACCGGATCTCCACCTACAC CCGGATTTGAACTGGGGAAA 55.0 50.0 57.9 58.1 1945-1978 bp 1.0 90.8
rpoB-trnC-GCA_p2 TACCGGATCTCCACCTACAC TTGAATGGCCGAATACTCCG 55.0 50.0 57.9 58.1 2356-2384 bp 0.555556 72.0
rpoB-trnC-GCA_p3 AGCCCAGATCCTTGCTTTTC TTGAATGGCCGAATACTCCG 50.0 50.0 58.2 58.1 2152-2180 bp 0.555556 71.7
rpoB-trnC-GCA_p4 GAGCCCAGATCCTTGCTTTT TTGAATGGCCGAATACTCCG 50.0 50.0 58.2 58.1 2153-2181 bp 0.555556 71.7
rpoB-trnC-GCA_p5 GCCCAGATCCTTGCTTTTCT TTGAATGGCCGAATACTCCG 50.0 50.0 58.2 58.1 2151-2179 bp 0.555556 71.7