Hotspot

atpH-atpI

LSC · 13322-14455 · 1134 bp

Back to Oxalis

Pi 0.0609
MarkerSeek score 80.20
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1134
variable_sites
175
pi
0.0609
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT CGGTGTAGAAGTAGGCCAAC 45.0 55.0 58.0 58.0 1839-1903 bp 1.0 91.0
atpH-atpI_p2 AAGAAAGAAAGCGGTTCGGT CGGTGTAGAAGTAGGCCAAC 45.0 55.0 58.0 58.0 2217-2303 bp 1.0 90.7
atpH-atpI_p3 GAAAGCGGTTCGGTATGCTA CGGTGTAGAAGTAGGCCAAC 50.0 55.0 58.1 58.0 2211-2297 bp 1.0 90.3
atpH-atpI_p4 CCAGTGACCCAAGAAAACGA CGGTGTAGAAGTAGGCCAAC 50.0 55.0 58.0 58.0 2523-2624 bp 0.888889 86.9
atpH-atpI_p5 AAGAAAGAAAGCGGTTCGGT TAGGGGGTTTCCAAGTCCAT 45.0 50.0 58.0 57.9 2182-2200 bp 0.222222 51.5