Hotspot

trnK-UUU-trnQ-UUG

LSC · 4433-6274 · 1842 bp

Back to Oxalis

Pi 0.0573
MarkerSeek score 82.80
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1842
variable_sites
274
pi
0.0573
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AAAGCCGAGTACTCTACCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1121-2066 bp 1.0 90.0
trnK-UUU-trnQ-UUG_p2 AAGCCGAGTACTCTACCGTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1120-2065 bp 1.0 90.0
trnK-UUU-trnQ-UUG_p3 GTTAGCCGCACTTAAAAGCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1135-2080 bp 1.0 89.3
trnK-UUU-trnQ-UUG_p4 CCGCACTTAAAAGCCGAGTA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 1130-2075 bp 1.0 89.1
trnK-UUU-trnQ-UUG_p5 TACCGTTGAGTTAGCAACCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1107-2052 bp 1.0 87.7