Hotspot

rpl16

LSC · 84080-85553 · 1474 bp

Back to Ottelia

Pi 0.0153

MarkerSeek score 59.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1474
variable_sites: 88
pi: 0.0153
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATCGATGACAACTGCTGCAT	AAATTGCAGGGCGTATCGAT	45.0	45.0	58.0	58.0	1992-2228 bp	1.0	87.7
rpl16_p2	ATCGATGACAACTGCTGCAT	GTCGAACACGAGGAATCGAA	45.0	50.0	58.0	58.0	2220-2456 bp	1.0	87.5
rpl16_p3	TCAATTCTCGAGCAATCGCA	AAATTGCAGGGCGTATCGAT	45.0	45.0	58.0	58.0	2047-2283 bp	0.947368	85.3
rpl16_p4	TCAATTCTCGAGCAATCGCA	GTCGAACACGAGGAATCGAA	45.0	50.0	58.0	58.0	2275-2511 bp	0.947368	85.2
rpl16_p5	TCAATTCTCGAGCAATCGCA	TTCCCCTACAGACCATTCGT	45.0	50.0	58.0	58.1	1984-2220 bp	0.842105	80.4