Hotspot

ccsA

SSC · 116717-117685 · 969 bp

Back to Ottelia

Pi 0.00837

MarkerSeek score 46.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 39
pi: 0.00837
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	CAGGCTGTGATAGAACGCAT	50.0	50.0	57.9	58.1	1813-1838 bp	1.0	82.7
ccsA_p2	TAGACACGCTGCTCTTAGGA	TGGGCCACGAGAACTATTTG	50.0	50.0	57.9	57.9	1494-1519 bp	1.0	82.7
ccsA_p3	GGTGAAATGGTAGACACGCT	CAGGCTGTGATAGAACGCAT	50.0	50.0	57.9	58.1	1824 bp	0.105263	43.0
ccsA_p4	GGTGAAATGGTAGACACGCT	TGGGCCACGAGAACTATTTG	50.0	50.0	57.9	57.9	1505 bp	0.105263	42.6
ccsA_p5	GGTGAAATGGTAGACACGCT	GGGCCACGAGAACTATTTGT	50.0	50.0	57.9	57.9	1504 bp	0.105263	42.6