Hotspot

ccsA-ndhD

SSC · 117686-117915 · 230 bp

Back to Ottelia

Pi 0.0496
MarkerSeek score 66.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
230
variable_sites
34
pi
0.0496
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTTGGGGT GCTATTGTATCTAATTACTTTCATAGA 40.0 25.9 54.0 53.4 338-347 bp 1.0 48.5
ccsA-ndhD_p2 TGGATATGCTATTTTGGGGT AGCTATTGTATCTAATTACTTTCATAG 40.0 25.9 54.0 53.4 339-348 bp 1.0 48.5
ccsA-ndhD_p3 TGGATATGCTATTTTGGGGTT GCTATTGTATCTAATTACTTTCATAGA 38.1 25.9 54.9 53.4 338-347 bp 1.0 48.5
ccsA-ndhD_p4 TTGGATATGCTATTTTGGGGT GCTATTGTATCTAATTACTTTCATAGA 38.1 25.9 54.9 53.4 339-348 bp 1.0 48.5
ccsA-ndhD_p5 TGGATATGCTATTTTGGGGTT AGCTATTGTATCTAATTACTTTCATAG 38.1 25.9 54.9 53.4 339-348 bp 1.0 48.5