Hotspot

rpl32-trnL-UAG

SSC · 115899-116535 · 637 bp

Back to Ottelia

Pi 0.0147

MarkerSeek score 62.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 637
variable_sites: 42
pi: 0.0147
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGCTTTTGAATGGCAGTTCC	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.3	57.9	1283-1381 bp	1.0	84.8
rpl32-trnL-UAG_p2	CGCTTTTGAATGGCAGTTCC	ACCACTGAGATTGCAAAGCA	50.0	45.0	58.3	58.0	1349-1447 bp	0.894737	80.8
rpl32-trnL-UAG_p3	CGCTTTTGAATGGCAGTTCC	GGGTAAAAATCGCACTTGGC	50.0	50.0	58.3	58.0	1271-1369 bp	0.842105	78.7
rpl32-trnL-UAG_p4	CGCTTTTGAATGGCAGTTCC	TACTTAGCGGAAAATGCCCC	50.0	50.0	58.3	58.0	1179-1197 bp	0.473684	58.1
rpl32-trnL-UAG_p5	CGCTTTTGAATGGCAGTTCC	AGCGTGTCTACCATTTCACC	50.0	50.0	58.3	57.9	836 bp	0.105263	41.1