Hotspot

psaJ-rpl33

LSC · 69962-70367 · 406 bp

Back to Ottelia

Pi 0.0233

MarkerSeek score 63.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 406
variable_sites: 46
pi: 0.0233
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	GTTACCCACACGGTTCTGTT	50.0	50.0	58.1	58.0	687-706 bp	1.0	87.7
psaJ-rpl33_p2	ACCCGTGACATTTTGTACCC	GTTACCCACACGGTTCTGTT	50.0	50.0	58.1	58.0	1131-1161 bp	1.0	86.3
psaJ-rpl33_p3	CCCGTGACATTTTGTACCCA	GTTACCCACACGGTTCTGTT	50.0	50.0	58.1	58.0	1130-1160 bp	1.0	86.3
psaJ-rpl33_p4	ACAGGATTTGAACCCGTGAC	GTTACCCACACGGTTCTGTT	50.0	50.0	58.1	58.0	1142-1172 bp	1.0	86.2
psaJ-rpl33_p5	ATCCCTCGTTACTGCCCATA	GTTACCCACACGGTTCTGTT	50.0	50.0	57.9	58.0	1184-1214 bp	1.0	86.0