Hotspot

ycf4-cemA

LSC · 62966-63957 · 992 bp

Back to Ottelia

Pi 0.0169

MarkerSeek score 62.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 67
pi: 0.0169
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	TAGAGAAATCCACCAGGGCA	45.0	50.0	58.1	58.0	1098-1115 bp	1.0	86.6
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	1096-1113 bp	1.0	86.3
ycf4-cemA_p3	ACAAAAAGCTGCGGAATTGG	TAGAGAAATCCACCAGGGCA	45.0	50.0	57.8	58.0	1114-1131 bp	1.0	86.0
ycf4-cemA_p4	ACAAAAAGCTGCGGAATTGG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	1112-1129 bp	1.0	85.7
ycf4-cemA_p5	TGCGGAATTGGCCTATTTCT	TAGAGAAATCCACCAGGGCA	45.0	50.0	57.6	58.0	1105-1122 bp	1.0	83.9