Hotspot

ndhC-trnV-UAC

LSC · 52649-53898 · 1250 bp

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Pi 0.0202
MarkerSeek score 71.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1250
variable_sites
103
pi
0.0202
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCCGGAAATTACAAATGCCA CGGTTCAAGTCCGTATAGCC 42.9 55.0 58.2 58.2 1229-1461 bp 0.947368 77.2
ndhC-trnV-UAC_p2 TCCCGGAAATTACAAATGCCA GGTTCAAGTCCGTATAGCCC 42.9 55.0 58.2 57.8 1228-1460 bp 0.947368 76.6
ndhC-trnV-UAC_p3 CCCGGAAATTACAAATGCCA CGGTTCAAGTCCGTATAGCC 45.0 55.0 56.7 58.2 1228-1460 bp 0.947368 76.1
ndhC-trnV-UAC_p4 CCCGGAAATTACAAATGCCA GGTTCAAGTCCGTATAGCCC 45.0 55.0 56.7 57.8 1227-1459 bp 0.947368 75.4
ndhC-trnV-UAC_p5 TCCCGGAAATTACAAATGCCA AGCCCAATGCAACAAAATGC 42.9 45.0 58.2 58.5 1321-1553 bp 0.947368 74.3