Hotspot

trnT-UGU-trnL-UAA

LSC · 48350-49243 · 894 bp

Back to Ottelia

Pi 0.0197
MarkerSeek score 68.80
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
894
variable_sites
67
pi
0.0197
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 874-959 bp 1.0 88.6
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC ACCAATTTCGCCATATCCCC 55.0 50.0 58.2 58.0 905-990 bp 1.0 88.2
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 882-967 bp 1.0 88.1
trnT-UGU-trnL-UAA_p4 GAGTCGAACCGATGACCATC TAGCGTCTACCAATTTCGCC 55.0 50.0 58.2 58.1 913-998 bp 1.0 87.7
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG ATCCAATCAAGTCCGTAGCG 55.0 50.0 57.9 57.8 897-982 bp 1.0 86.7