Hotspot

petN-psbM

LSC · 29538-30875 · 1338 bp

Back to Ottelia

Pi 0.0209

MarkerSeek score 65.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1338
variable_sites: 109
pi: 0.0209
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CGGTATCGGTTTTGGCTACA	TTTTATTGGTTGGATGCGCG	50.0	45.0	58.0	57.7	2401-2542 bp	0.684211	72.2
petN-psbM_p2	CTCTGCTCCTATTGGCCAAG	TTTTATTGGTTGGATGCGCG	55.0	45.0	58.0	57.7	2292-2433 bp	0.684211	72.0
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TTTTATTGGTTGGATGCGCG	50.0	45.0	58.2	57.7	1881-2034 bp	0.684211	71.1
petN-psbM_p4	AGTAGGTAGGGGTATTCCGC	TTTTATTGGTTGGATGCGCG	55.0	45.0	58.0	57.7	2157-2300 bp	0.421053	59.3
petN-psbM_p5	AGTAGGTAGGGGTATTCCGC	CGCGAGCAATGGAATAGGAA	55.0	50.0	58.0	58.4	2141-2284 bp	0.421053	58.1