Hotspot

rpoB-trnC-GCA

LSC · 27590-28797 · 1208 bp

Back to Ottelia

Pi 0.0163

MarkerSeek score 66.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1208
variable_sites: 79
pi: 0.0163
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ACTCTTCTGTCAAGCCCTGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.3	58.5	1370-1409 bp	1.0	80.1
rpoB-trnC-GCA_p2	ACTCTTCTGTCAAGCCCTGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.3	57.5	1345-1384 bp	1.0	79.8
rpoB-trnC-GCA_p3	ACTCTTCTGTCAAGCCCTGA	CCTTACCACTTGGCCATGTC	50.0	55.0	58.3	58.5	1324-1363 bp	1.0	79.8
rpoB-trnC-GCA_p4	ACTCTTCTGTCAAGCCCTGA	TATTGAGCTGATCAGGCGAC	50.0	50.0	58.3	57.5	1385-1424 bp	1.0	79.7
rpoB-trnC-GCA_p5	AACTCTTCTGTCAAGCCCTG	GCGACACCCAGATTTGAACT	50.0	50.0	57.4	58.5	1371-1410 bp	1.0	77.8