Hotspot

rps16-trnQ-UUG

LSC · 6081-6923 · 843 bp

Back to Ottelia

Pi 0.0203

MarkerSeek score 69.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 70
pi: 0.0203
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	TGGTCCTGTTATTCGGAGGT	45.0	50.0	58.1	58.1	891-989 bp	1.0	88.5
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	876-974 bp	1.0	88.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	880-978 bp	1.0	83.7
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	TGGTCCTGTTATTCGGAGGT	50.0	50.0	57.2	58.1	883-981 bp	1.0	83.4
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	868-966 bp	1.0	83.3