Hotspot

trnK-UUU-rps16

LSC · 4195-4808 · 614 bp

Back to Ottelia

Pi 0.0206
MarkerSeek score 69.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
614
variable_sites
56
pi
0.0206
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGGGCTCAACCTACAGAAAC 50.0 55.0 58.2 58.2 733-799 bp 1.0 85.2
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGGGCTCAACCTACAGAAAC 50.0 55.0 58.2 58.2 732-798 bp 1.0 85.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GCTCAACCTACAGAAACGGT 50.0 50.0 58.2 57.8 729-795 bp 0.947368 83.0
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT GCTCAACCTACAGAAACGGT 50.0 50.0 58.2 57.8 730-796 bp 0.947368 82.9
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GCTCAACCTACAGAAACGGT 50.0 50.0 58.3 57.8 739-805 bp 0.947368 81.9