Hotspot

trnH-GUG-psbA

LSC · 77-343 · 267 bp

Back to Ottelia

Pi 0.0192

MarkerSeek score 63.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 27
pi: 0.0192
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CAATCCACCGCCTTAATCCA	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	351-365 bp	1.0	88.7
trnH-GUG-psbA_p2	AATCCACCGCCTTAATCCAC	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	350-364 bp	1.0	88.7
trnH-GUG-psbA_p3	ACAATCCACCGCCTTAATCC	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	352-366 bp	1.0	88.6
trnH-GUG-psbA_p4	CAATCCACCGCCTTAATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	389-403 bp	1.0	88.2
trnH-GUG-psbA_p5	AATCCACCGCCTTAATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	388-402 bp	1.0	88.2