Hotspot

rps2

LSC · 31652-32362 · 711 bp

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Pi 0.00985
MarkerSeek score 64.40
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
LSC
length_bp
711
variable_sites
7
pi
0.00985
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 CCGTAATGGAATGGTAGGATGA AAATCTACGCAGTTGCCCAA 45.5 45.0 57.4 58.1 810 bp 1.0 59.0
rps2_p2 CCGTAATGGAATGGTAGGATGA ACGCAGTTGCCCAAGAATTA 45.5 45.0 57.4 58.1 804 bp 1.0 59.0
rps2_p3 CCGTAATGGAATGGTAGGATGA TACGCAGTTGCCCAAGAATT 45.5 45.0 57.4 58.1 805 bp 1.0 59.0
rps2_p4 TCCGTAATGGAATGGTAGGATG ACGCAGTTGCCCAAGAATTA 45.5 45.0 57.4 58.1 805 bp 1.0 59.0
rps2_p5 TCCGTAATGGAATGGTAGGATG TACGCAGTTGCCCAAGAATT 45.5 45.0 57.4 58.1 806 bp 1.0 59.0