Hotspot

matK

LSC · 1683-3218 · 1536 bp

Back to Otatea

Pi 0.00391
MarkerSeek score 60.90
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1536
variable_sites
6
pi
0.00391
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 TCAGAATGGAAAACGTGAAAGT CAACACCCTGTTCTGACCAT 36.4 50.0 56.5 57.7 1699 bp 1.0 50.0
matK_p2 TCAGAATGGAAAACGTGAAAGT AATGCAACACCCTGTTCTGA 36.4 45.0 56.5 57.6 1703 bp 1.0 49.3
matK_p3 TCAGAATGGAAAACGTGAAAGT ATGCAACACCCTGTTCTGAC 36.4 50.0 56.5 58.4 1702 bp 1.0 49.1
matK_p4 ACATTTCAGAATGGAAAACGTGA CAACACCCTGTTCTGACCAT 34.8 50.0 57.2 57.7 1704 bp 1.0 47.9
matK_p5 AGGGAACATTTCAGAATGGA CAACACCCTGTTCTGACCAT 40.0 50.0 54.2 57.7 1709 bp 1.0 47.6