Hotspot

petA-psbJ

LSC · 62712-63699 · 988 bp

Back to Otatea

Pi 0.00304
MarkerSeek score 60.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
988
variable_sites
3
pi
0.00304
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCGTCCAAGGCCTTTTATTC TCCTCTTTGGCTGATAGGTACT 50.0 45.5 57.4 58.0 1197-1198 bp 1.0 59.7
petA-psbJ_p2 GCGTCCAAGGCCTTTTATTC CCTCTTTGGCTGATAGGTACTG 50.0 50.0 57.4 57.9 1196-1197 bp 1.0 59.3
petA-psbJ_p3 GCGTCCAAGGCCTTTTATTC TTCCTCTTTGGCTGATAGGT 50.0 45.0 57.4 55.8 1198-1199 bp 1.0 58.4
petA-psbJ_p4 GCGTCCAAGGCCTTTTATTC ACGGTTCATATTCTGGATTGGG 50.0 45.5 57.4 58.2 1132-1133 bp 1.0 58.4
petA-psbJ_p5 GCGTCCAAGGCCTTTTATTC CGGTTCATATTCTGGATTGGGT 50.0 45.5 57.4 58.2 1131-1132 bp 1.0 58.4